| PFKFB3

PFKFB3 is a gene that encodes the 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 enzyme.It is one of 4 tissue-specific PFKFB isoenzymes identified currently (PFKFB1-4).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.2	0
OD	2.4	1.69	1.29	0.9	0.61	0.43	0.31	0.09
Corrected OD	2.31	1.6	1.2	0.81	0.52	0.34	0.22	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat PFKFB3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	80-101	96
Plasma(n=8)	92-106	102
Cell Culture Supernates(n=8)	96-108	106

 

| Linearity

Samples were spiked with high concentrations of Rat PFKFB3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-111
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	89-108	105-115
	Average (%)	95	97	108